Pipes Good Purity Reagent Grade 5625-37-6 1, 4-Piperazinebis Ethanesulphonic Acid

Product Description

Product Description

 

PIPES, The chemical name piperazine-N, N '- bis (2-ethylsulfonic acid), CAS number 5625-37-6, is a zwitterionic biological buffer belonging to the Good's buffer series. Its molecular weight is 302.37. The effective buffering range of PIPES is pH 6.1-7.5, with a pKa of 6.8 at 25 ºC, which is close to the physiological pH environment. It is insoluble in water and needs to be dissolved with NaOH solution. PIPES does not chelate metal ions and is commonly used in biochemical experiments such as reaction systems containing metal ions, cell culture, and electron microscopy fixatives.

 

Packaging Specification : Plastic bottles, 500g/bottle

 

Product Parameters

Product name	Pipes
Abbreviation	PIPES	Molecular formula	C8H18N2O6S2
CAS No	5625-37-6	Molecular weight	302.40
Appearance	White crystalline powder	Purity	>99%
Storage Conditions	Room temperature, light-proof and moisture-proof

usage method

PIPES is different from other buffering agents, such as BIS-TRIS and BICINE, as it cannot form stable compounds with many metal ions, making it very suitable as a buffering agent in metal ion solutions.

There are two pKa values for PIPES, namely 1pKa and 2.67pKa. 1pKa is 6.76 at ambient temperature of 25 ° C, close to physiological pH, and has a buffer range of 6.1-7.5, which is suitable for cell culture work. 2.67pKa has a buffer range of 1.5-3.5 at 25 ° C.
 

Application

PIPES can be used to purify tubulin by cellulose phosphate chromatography and recombinant GTP by gel filtration. It can bind proteins ARF1 and ARF2 to crystallize transketolase from Escherichia coli as a buffer solution. However, it is worth noting that PIPES is not suitable for redox systems due to its ability to form free radicals.
 
 

Our Advantages

 

1. The fixation of glutaraldehyde in animal and plant tissue samples can lead to lipid loss with significant morphological changes. When using PIPES buffered glutaraldehyde fixative, lipid loss and artifacts are minimized.

2. If immobilized in oxalate buffered glutaraldehyde for supercellular chemical fixation, it is possible to selectively lose the activity of alkaline phosphatase. When using PIPES as a buffer, the activity retention rate will be significantly improved.

3. The binding of glutaraldehyde and formaldehyde in PIPES buffer can achieve excellent immobilization of fungal spores under fluorescence and electron microscopes.
 

 

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Company Profile

 

Hubei New Desheng Material Technology Co., Ltd. specializes in the production of blood collection tube additives, chemiluminescent reagents, biological buffers, chromogenic substrates, enzyme preparations, antigen antibodies and other biochemical reagents. Founded in 2005, Desheng has a professional R&D team and advanced production equipment!
 

 

FAQ

 

Question:Who are we?
Answer:We are Hubei Xindesheng Material Technology Co., Ltd., a professional manufacturer of in vitro diagnostic reagent raw materials.

Question:Where is the company?
Answer:Huarong District, Ezhou City, Hubei Province, is adjacent to Wuhan City.Welcome to visit the company!

Question:What is the delivery time?
Answer:It is determined based on the inventory status of the product and the production and delivery status on that day. Generally, if there is stock available, shipment will be arranged on the day of placing the order.

Question:How to choose the transportation method?
Answer:Determine based on the urgency of the customer's purchase of products and needs. When the quantity of products is low and the value is high, it is recommended to choose air freight or international express delivery; When there is a large quantity of products, choosing sea or land transportation is more cost-effective.

Question:What are the payment methods available?
Answer:T/T,LC, etc

Question:What are the regular packaging options?
Answer:25kg/barrel, 20kg/barrel