CAS 7365-45-9 How to Make Hepes Buffer for Cell Culture Medium

• Customization: Available
• CAS No.: 7365-45-9
• Formula: C8h18n2o4s
• Model NO.: Hepes Buffer
• EINECS: 230-907-9
• Classification: Biochemical Reagents
• Grade: AR
• Specific Usage: For Biological Purpose, For Tissue Medium Purpose, Technical Grade, Pratical Use, Pro Analysis, Biological Buffer
• Content: Purity Above 99%
• Usage: Laboratory Reagents, Diagnostic Reagents
• Source: Dry Powder
• Habit Appellation: Hepes Buffer
• Application: Scientific Research, Health
• Property: Biochemical Reagent
• Colour: White
• Storage Conditions: Room Temp
• Form: Powder
• Water Solubility: Soluble
• Purity: Above 99%
• Molecular Weight: 238.3
• Use to: Biological Buffer
• OEM Packing: Yes
• Manufacturer: Hubei Xindesheng
• Transport Package: Carton
• Specification: 500g/bottle
• Trademark: DeSheng
• Origin: Ezhou City, Hubei Province, China
• Production Capacity: 5 Tons Per Month

Product Description

Packaging Specification : Plastic bottles, 500g/bottle

Basic Information
   

tems	Specifications
Appearance	White crystalline powder
Purity	≥99%
Loss on drying	≤1.0%
Heavy metals	≤0.001%
PH	5.0-6.5
Fe	≤0.0005%

Product Introduction
HEPES is an important hydrogen ion buffer that has good buffering ability in the pH range of 6.8 to 8.2, and can control a constant Chemicalbook pH for a long time. A concentration of 10-50mmol/L and a general culture medium containing 20mmol/L of 4-hydroxyethyl ethanesulfonic acid can provide good buffering ability and have no toxic effects on cells.

usage method

(1) Hepes buffer can be directly added to the prepared culture medium according to the required concentration, and then filtered for sterilization. 2.38 grams of HEPES are added to every 1000ml of culture medium, and after being dissolved, the pH is adjusted to 7.2 with lNNaOH. After filtering for sterilization, it is used. At this time, the concentration of HEPES is 10mmol/L

(2) It can also be prepared into 100x storage solution (lmol/L). Before use, Chemicalbook takes 99ml of culture solution and adds it to 1ml of storage solution. The final application concentration is still 10mmol/L. Preparation method of lmol/L (100x) hepes buffer solution: Dissolve 23.8g of HEPES in 90ml of double distilled water, adjust the pH to 7.5-8.0 with lNNaOH, and then dilute to 100ml with water. Filter and sterilize the solution, then divide into small bottles (2ml/bottle) and store at 4 ºC or -20 ºC
 

Application

HEPES  is a non-ionic amphoteric buffer with high polarity. It is inert to a variety of chemical reagents and enzymes, does not participate in and do not interfere with the biochemical reaction process of the Chemicalbook, and has no inhibitory effect on enzyme chemical reactions. Therefore, it can be specifically used for research on organelle and highly variable, pH sensitive proteins and enzymes.
 

 
Product Advantages

Purity ≥ 99%, good water solubility, stable process, and can ensure that the product appearance is pure white crystal powder.

Use to

Used as a biochemical reagent and biological buffer; Reaction buffer, pre hybridization buffer, and hybridization buffer for separating and analyzing RNA nuclear components; It is used for RNA and T4RNA, molecular biology grade for RNA3 '- terminal labeling with T4 RNA ligase, separation and analysis of reaction buffer components of nuclear RNA, components of pre hybridization buffer buffer buffer buffer buffer buffer buffer buffer buffer buffer buffer buffer, cell cell adhesion, short-term cell aggregation culture, buffer solution used for cleaning tissue and cells



Company Introduction
Hubei New Desheng Material Technology Co., Ltd. specializes in the production of blood collection tube additives, chemiluminescent reagents, biological buffers, chromogenic substrates, enzyme preparations, antigen antibodies and other biochemical reagents. Founded in 2005, Desheng has a professional R&D team and advanced production equipment!