| Customization: | Available |
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| CAS No.: | 4432-31-9 |
| Formula: | C6h13no4s |
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MES is a zwitterionic morpholine buffer suitable for pH ranges of 5.5-6.7, commonly used as a running buffer in cell culture media and electrophoresis. It is one of the Good's buffering agents and possesses all the characteristics and advantages of Good's buffering agents.
| Abbreviation | MOPS | CAS No | 1132-61-2 |
| Molecular formula | C7H15NO4S | Molecular weight | 209.27 |
| Purity | ≥99% | Appearance | White crystal powder |
| pKa | 7.2 | pH range | 6.5-7.9 |
Packaging Specification : Plastic bottles, 500g/bottle
The pka value is in the neutral range; The Pka value is not affected by temperature and changes; The powder has good water solubility and is not easily soluble in other solvents; Difficult to chelate with salt ions; Good use effect and strong stability; There is no significant light absorption in the ultraviolet and visible light bands; Easy to synthesize
1. Prepare 800 mL of dH2O in a suitable container.
As anion buffer, MES buffer is suitable for cation exchange chromatography and hydroxyapatite chromatography; It can be used for gel filtration chromatography; Can be used for cellulose phosphate column chromatography; Can be used for hydrophobic substance interaction chromatography; Can be used for SDS-PAGE; Can be used for fluorescence microscopy; Suitable as a non coordinating buffer in metal ion solutions; Due to the fact that MES buffer does not form free radicals, it is suitable for the study of redox processes; Because it will not be metabolized by bacteria and eukaryotic cell, MES buffer can be used to prepare culture media, such as low concentration plant cell culture media and mammalian cell culture media; Suitable for most toxin research; Used for in vitro dissolution test of liposomes; The interaction between MES buffer and the peptide skeleton of bovine serum albumin can enhance the stability of the protein
Each buffer developed has a specific buffer capacity and buffer range. Buffer capacity is the amount of acid or base that can be added before a significant change in pH begins. It can also be defined as the amount of strong acid or strong base that must be added to change the pH value of one liter of solution by one pH unit. After adding acidic or alkaline components, it plays a role in resisting pH changes. Maintain a relatively stable pH value of the solution by neutralizing a small amount of added acid or base.
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