Customization: | Available |
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CAS No.: | 7365-82-4 |
Formula: | C4h10n2o4s |
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Bis Tris is the abbreviation for bis (2-hydroxyethyl) aminomethane, with a molecular formula of C8H19NO5 and a molecular weight of 209.24 g/mol. It combines functional groups from two different structural families: the bis (2-hydroxyethyl) amine family and the Tris family. Its pKa value is 6.46, making it an ideal buffer in the pH range of 5.8 to 7.2. This makes Bis Tris widely applicable in many biological and biochemical experiments.
In addition, Bis Tris is easily soluble in water, forming a colorless and transparent solution. It can maintain good buffering capacity at different temperatures and ionic strengths, and has good chemical stability. These properties make Bis Tris an ideal buffer choice in electrophoresis experiments.
Bis Tris is widely used in multiple fields of biochemistry and molecular biology, such as:
1. Anion exchange chromatography: As a buffer, Bis Tris can maintain the stability of pH in the chromatographic column, ensuring the accuracy of separation efficiency.
2. Electrophoresis buffer: In gel electrophoresis and sample buffer, Bis Tris can provide a stable pH environment and improve the resolution of electrophoresis.
3. Running buffer of gel electrophoresis: used together with ACES, Bis Tris/ACES buffer system performs well in electrophoresis experiments, and is especially suitable for the separation of proteins and nucleic acids.
4. Metal complexes: Bis Tris can form strong complexes with Pb2+and Cu2+, but has weak interactions with other common metal ions, making it particularly advantageous in certain metal research.
However, it should be noted that Bis Tris is not suitable for use with BCA detection and may interfere with the detection results. Therefore, when selecting a buffer solution, specific detection methods and requirements in the experiment need to be considered.
Assay Percent Range | 99% |
Formula Weight | 209.24 |
Physical Form | Crystalline Powder |
Percent Purity | ≥99% |
Grade | Biochemical |
Packaging | bottle |
pH | 5.8-7.2(at 25ºC) |
Color | White |
Quantity | 1000g |
Chemical Name or Material | Bis Tris Buffer |
Packaging Specification : Plastic bottles, 500g/bottle,25KG/Cardboard bucket
Hubei New Desheng Material Technology Co., Ltd. specializes in the production of blood collection tube additives, chemiluminescent reagents, biological buffers, chromogenic substrates, enzyme preparations, antigen antibodies and other biochemical reagents. Founded in 2005, Desheng has a professional R&D team and advanced production equipment!
Question:Who are we?
Answer:We are Hubei Xindesheng Material Technology Co., Ltd., a professional manufacturer of in vitro diagnostic reagent raw materials.
Question:Where is the company?
Answer:Huarong District, Ezhou City, Hubei Province, is adjacent to Wuhan City.Welcome to visit the company!
Question:What is the delivery time?
Answer:It is determined based on the inventory status of the product and the production and delivery status on that day. Generally, if there is stock available, shipment will be arranged on the day of placing the order.
Question:How to choose the transportation method?
Answer:Determine based on the urgency of the customer's purchase of products and needs. When the quantity of products is low and the value is high, it is recommended to choose air freight or international express delivery; When there is a large quantity of products, choosing sea or land transportation is more cost-effective.
Question:What are the payment methods available?
Answer:T/T,LC, etc
Question:What are the regular packaging options?
Answer:500g/bottle, plastic bottle or 25kg/bucket, cardboard bucket.