in Vitro Diagnostic Kit Raw Material Enzyme Preparation Neuraminidase, Nrh

• CAS No.: No
• Formula: No
• EINECS: No
• Classification: Biological Agents
• Grade: AR
• Specific Usage: For Biological Purpose
• Model NO.: DS0220
• Content: Standard
• Usage: Laboratory Reagents, Analytical Reagents, Diagnostic Reagents
• Source: Composition
• Habit Appellation: Glucose Dehydrogenase, Gdh
• Application: Scientific Research, Health
• Property: Biochemical Reagent
• Storage: Low Temperature Storage
• Use to: in Vitro Diagnostic Kit
• Manufactor: Hubei New Desheng Material Technology Co., Ltd
• Dosage Form: White Freeze-Dried Powder
• Transport Package: Refrigerated Transport
• Specification: 10mg/bottle
• Trademark: DeSheng
• Origin: Ezhou City, Hubei Province, China
• Production Capacity: 50g Per Month

Product Description

Basic information
 

Dosage form:	White freeze-dried powder, or liquid	Molecular weight:	About58 kDa (SDS-PAGE detection)
Save buffer:	50mM phosphate buffer, pH 6.8	Purity	≥ 90% (SDS-PAGE detection)
Enzyme activity:	100U/mg protein	Transportation Conditions:	Low temperature, ice bag
Source:	Gene recombination expression	Safety tips:	Not applicable for human experiments
storage condition:	Store at 4 ºC for about a year (after packaging according to dosage, store at -20 ºC or -80 ºC for a longer period of time and try to avoid repeated freeze-thaw)

Product Introduction

Neuraminidase (NRH, EC 3.2.1.18) is commonly known as acetylneuraminidase (sialidase). This enzyme catalyzes the hydrolysis of glycoproteins and oligosaccharides α 2-3, α 2-6 and α N-acetylneuraminic acid residues connected by 2-8 bonds.

Biochemical research

The concentration of sialic acid (SA) in the blood has been regarded as an important indicator for monitoring the presence and development of malignant tumors, and has been widely used in the diagnosis and monitoring of various tumors (such as lung cancer, gastric cancer, colorectal cancer, liver cancer, ovarian cancer, etc.). Enzymatic detection of sialic acid has the advantages of speed, simplicity, high sensitivity, and ease of automation. NRH is a very important enzyme in the sialic acid enzyme detection kit, and as the first step enzyme in initiating the entire chemical reaction of the kit, it has a very important role and significance.




The relationship between enzyme preparations, new Trinder's reagents, and Good's buffer

There are many principles of in vitro diagnostic kits, and the most common one is a diagnostic kit based on the Trinder reaction principle (also known as enzyme method), which can be used for the detection of uric acid, creatinine, blood sugar, cholesterol, triglycerides, etc. in blood tests. This diagnostic kit typically includes important components such as enzymes, new Trinder's reagents, and Good's buffer.

By adding the new Trinder's reagent to the test substance, a color reaction occurs, and the value of the test item is calculated through absorbance measurement. For example, the values of blood sugar and uric acid in serum. The new Trinder's reagent has the advantages of high water solubility, UV absorption of color reaction products>540nm, wide pH range requirements for color reactions, and high sensitivity for color reactions.
 

The use of enzyme preparations in the Trinder reaction is due to the presence of too many substances in the serum, and the specificity of enzyme preparations. For example, adding glucose oxidase will only catalyze the oxidation of blood sugar to generate hydrogen peroxide, without catalyzing the oxidation of uric acid and other substances present in the serum, ensuring the accuracy of the detection results as much as possible. However, pH value has a significant impact on enzyme activity, and each enzyme has its own high activity pH range. At this point, Good's buffer is needed to adjust the pH value.

Different Good's buffers can provide suitable buffer intervals for different enzymes. Good's buffer has the advantages of high water solubility, bottom cell membrane permeability, stable acid-base dissociation constant, low metal chelating ability, high chemical stability, etc. In summary, enzyme preparations, new Trinder's reagents, and Good's buffer complement each other in the diagnostic kit, making corresponding contributions to the precise detection of diseases.
 

Company Introduction
Hubei New Desheng Material Technology Co., Ltd. specializes in the production of blood collection tube additives, chemiluminescent reagents, biological buffers, chromogenic substrates, enzyme preparations, antigen antibodies and other biochemical reagents. Founded in 2005, Desheng has a professional R&D team and advanced production equipment!