Cholesterol Oxidase Cod 9028-76-6 Diagnostic Reagent Kit Raw Materials

• Customization: Available
• CAS No.: No
• Formula: No
• Model NO.: Cholesterol Oxidase
• EINECS: No
• Classification: Biological Agents
• Grade: AR
• Specific Usage: For Biological Purpose
• Content: Standard
• Usage: Laboratory Reagents, Analytical Reagents, Diagnostic Reagents
• Source: Composition
• Habit Appellation: S-Adenosylmethionine Synthetase Sams
• Application: Scientific Research, Health
• Property: Biochemical Reagent
• Storage: Low Temperature Storage
• Use to: in Vitro Diagnostic Kit
• Manufactor: Hubei New Desheng Material Technology Co., Ltd
• Dosage Form: White Freeze-Dried Powder
• Transport Package: Refrigerated Transport
• Specification: 10mg/bottle
• Trademark: DeSheng
• Origin: Ezhou City, Hubei Province, China
• Production Capacity: 50g Per Month

Product Description

Product Description

Total cholesterol (TC) in serum includes two parts: free cholesterol (FC) and cholesterol ester (CE). Cholesterol esters in serum can be hydrolyzed into free cholesterol and free fatty acids (FFA) by cholesterol esterase, and cholesterol generates △ 4-cholesterone and hydrogen peroxide under the oxidation of cholesterol oxidase.


 

Product Parameters

 

Dosage form:	White freeze-dried powder, or liquid	Molecular weight:	(SDS-PAGE detection)
Save buffer:	PBS,pH7.4	Purity	≥ 90% (SDS-PAGE detection)
Enzyme activity:	100 U/mg	Transportation Conditions:	Low temperature, ice bag
Source:	Gene recombination expression	Safety tips:	Not applicable for human experiments
Storage condition	Freeze dried powder should be stored at 4 ºC for one year and -20 ºC for two years (it is recommended to store separately and avoid repeated freeze-thaw as much as possible)

 

Scope of application

1. Clinical biochemical analysis: used as a key component in in vitro diagnostic kits to detect total cholesterol or high-density lipoprotein cholesterol (HDL-C) levels in blood or other samples. Preparation of raw enzymes for blood glucose and blood lipid reagents, as well as TCHO, HDL, and LDL reagents.

2. Serum cholesterol detection kit: used to determine the total cholesterol level in serum.

3. High density lipoprotein (HDL) cholesterol detection kit: used to determine the level of high-density lipoprotein cholesterol in serum.

4. Low density lipoprotein (LDL) cholesterol detection kit: used to determine the level of low-density lipoprotein cholesterol in serum.

5. Cholesterol detection in food and beverages: used to detect the cholesterol content in food and beverages to ensure that products meet food safety standards.

6. Research purpose: In biochemical and molecular biology research, cholesterol oxidase is also used to study cholesterol metabolism and related biochemical pathways.



The relationship between enzyme preparations, new Trinder's reagents, and Good's buffer

There are many principles of in vitro diagnostic kits, and the most common one is a diagnostic kit based on the Trinder reaction principle (also known as enzyme method), which can be used for the detection of uric acid, creatinine, blood sugar, cholesterol, triglycerides, etc. in blood tests. This diagnostic kit typically includes important components such as enzymes, new Trinder's reagents, and Good's buffer.

By adding the new Trinder's reagent to the test substance, a color reaction occurs, and the value of the test item is calculated through absorbance measurement. For example, the values of blood sugar and uric acid in serum. The new Trinder's reagent has the advantages of high water solubility, UV absorption of color reaction products>540nm, wide pH range requirements for color reactions, and high sensitivity for color reactions.
 

The use of enzyme preparations in the Trinder reaction is due to the presence of too many substances in the serum, and the specificity of enzyme preparations. For example, adding glucose oxidase will only catalyze the oxidation of blood sugar to generate hydrogen peroxide, without catalyzing the oxidation of uric acid and other substances present in the serum, ensuring the accuracy of the detection results as much as possible. However, pH value has a significant impact on enzyme activity, and each enzyme has its own high activity pH range. At this point, Good's buffer is needed to adjust the pH value.

Different Good's buffers can provide suitable buffer intervals for different enzymes. Good's buffer has the advantages of high water solubility, bottom cell membrane permeability, stable acid-base dissociation constant, low metal chelating ability, high chemical stability, etc. In summary, enzyme preparations, new Trinder's reagents, and Good's buffer complement each other in the diagnostic kit, making corresponding contributions to the precise detection of diseases.
 

Company Introduction
Hubei New Desheng Material Technology Co., Ltd. specializes in the production of blood collection tube additives, chemiluminescent reagents, biological buffers, chromogenic substrates, enzyme preparations, antigen antibodies and other biochemical reagents. Founded in 2005, Desheng has a professional R&D team and advanced production equipment!