Chaps Buffer Preparation Non Denaturing Zwitterion Detergent for Membrane Biochemistry

• CAS No.: 75621-03-3
• Formula: C32h58n2o7s
• EINECS: 616-246-2
• Classification: Biochemical Reagents
• Grade: AR
• Specific Usage: For Biological Purpose, For Tissue Medium Purpose, Technical Grade, Pratical Use, Pro Analysis, Biological Buffer
• Model NO.: CHAPS Buffer
• Content: Purity Above 99%
• Usage: Laboratory Reagents, Diagnostic Reagents
• Source: Dry Powder
• Habit Appellation: Chaps Buffer
• Application: Scientific Research, Health
• Property: Biochemical Reagent
• Colour: White
• Storage Conditions: Room Temp
• Form: Powder
• Water Solubility: Soluble
• Purity: Above 99%
• Molecular Weight: 614.88
• Melting Point: 156-158°c
• Density: 1.01 G/Ml at 20 ° C
• Use to: Biological Buffer
• OEM Packing: Yes
• Transport Package: Carton
• Specification: 500g/bottle
• Trademark: DeSheng
• Origin: Ezhou City, Hubei Province, China
• Production Capacity: 5 Tons Per Month

Product Description

Packaging Specification : Plastic bottles, 500g/bottle

Basic Information
  

CAS Number	75621-03-3
Molecular Formula	C32H58N2O7S
Molecular Weight	614.88
EINECS	616-246-2
Appearance	White Powder
Density	1.01g/cm3
Melting Point	156-158ºC
Solubility	H2O: 50 mg/mL at 20 °C, clear, colorless

 

Biological activity

CHAPS, a derivative of Cholic acid, is a zwitterion detergent that can dissolve membrane proteins. CHAPS is used to stabilize various protein DNA complexes and retain the biochemical activity of proteins in solution.

In vitro research

CHAPS(0.5%)isabletostabilizecomplexesbetweenDNAandDNA-bindingfactorssuchasAP-1,SPI,GATA-1andα-regulatedfactorISGF3,Chemicalbookandretainstheirbiochemicalactivity.CHAPScanpreventdissociationofmononucleosomesdilutedtosub-nanomolarconcentrations.
 

 
Use to

CHAPS is a non denaturing zwitterion detergent used in membrane biochemistry. It can be used to dissolve membrane proteins and interrupt protein-protein interactions. CHAPS has a low micelle molecular weight (6150) and a high critical micelle concentration (6-10mM), so it can be removed from the sample through dialysis. Chemicalbook is also suitable for dissolving proteins in isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non denaturing (urea free) isoelectric focusing and has been found to exhibit excellent resolution for certain subcellular preparations and plant proteins. Usually, the concentration between 2-4% (w/v) is used for isoelectric focusing gel.



Company Introduction
Hubei New Desheng Material Technology Co., Ltd. specializes in the production of blood collection tube additives, chemiluminescent reagents, biological buffers, chromogenic substrates, enzyme preparations, antigen antibodies and other biochemical reagents. Founded in 2005, Desheng has a professional R&D team and advanced production equipment!