Laboratory Reagent Mes Buffer for DNA and Rna Extraction Experiments

Product Description

Product Description

MES is a zwitterionic morpholine buffer suitable for pH ranges of 5.5-6.7, commonly used as a running buffer in cell culture media and electrophoresis. It is one of the Good's buffering agents and possesses all the characteristics and advantages of Good's buffering agents.
 

Product Parameters

 

Abbreviation	MOPS	CAS No	1132-61-2
Molecular formula	C7H15NO4S	Molecular weight	209.27
Purity	≥99%	Appearance	White crystal powder
pKa	7.2	pH range	6.5-7.9

 

    
Packaging Specification : Plastic bottles, 500g/bottle
 

Product advantages

 

The pka value is in the neutral range; The Pka value is not affected by temperature and changes; The powder has good water solubility and is not easily soluble in other solvents; Difficult to chelate with salt ions; Good use effect and strong stability; There is no significant light absorption in the ultraviolet and visible light bands; Easy to synthesize
 

How to prepare a solution

1. Prepare 800 mL of dH2O in a suitable container.

2. Add 97.62 grams of MES free acid to the dH2O solution.

3. The initial pH value of 0.5M MES solution is 3.23. For 1 L of 0.5M MES, 13.6 ml of 10N NaOH is required to adjust the pH to 6.0;

4. Add dH2O until the volume is 1L.

Storage: MES solution can be stored for several months at 2-8 ºC. It is recommended to use a 0.2 micron filter membrane for filtration and sterilization. (Substances containing sulfate ions are not easily sterilized at high temperatures) After high-temperature sterilization, MES will show a light yellow color. It is not clear what the light yellow degradation product is, but the pH will not change significantly.

Application

As anion buffer, MES buffer is suitable for cation exchange chromatography and hydroxyapatite chromatography; It can be used for gel filtration chromatography; Can be used for cellulose phosphate column chromatography; Can be used for hydrophobic substance interaction chromatography; Can be used for SDS-PAGE; Can be used for fluorescence microscopy; Suitable as a non coordinating buffer in metal ion solutions; Due to the fact that MES buffer does not form free radicals, it is suitable for the study of redox processes; Because it will not be metabolized by bacteria and eukaryotic cell, MES buffer can be used to prepare culture media, such as low concentration plant cell culture media and mammalian cell culture media; Suitable for most toxin research; Used for in vitro dissolution test of liposomes; The interaction between MES buffer and the peptide skeleton of bovine serum albumin can enhance the stability of the protein

 

 
Product Advantages

Purity ≥ 99%, good water solubility, stable process, and can ensure that the product appearance is pure white crystal powder.



 

Related products

 

Each buffer developed has a specific buffer capacity and buffer range. Buffer capacity is the amount of acid or base that can be added before a significant change in pH begins. It can also be defined as the amount of strong acid or strong base that must be added to change the pH value of one liter of solution by one pH unit. After adding acidic or alkaline components, it plays a role in resisting pH changes. Maintain a relatively stable pH value of the solution by neutralizing a small amount of added acid or base.
Desheng is a manufacturer of industrial grade biological buffering agents, currently producing dozens of buffering agents that can be used in various industries.
 

Company Profile

 

Company Introduction
Hubei New Desheng Material Technology Co., Ltd. specializes in the production of blood collection tube additives, chemiluminescent reagents, biological buffers, chromogenic substrates, enzyme preparations, antigen antibodies and other biochemical reagents. Founded in 2005, Desheng has a professional R&D team and advanced production equipment!

 

 

FAQ

 

Question:Who are we?
Answer:We are Hubei Xindesheng Material Technology Co., Ltd., a professional manufacturer of in vitro diagnostic reagent raw materials.

Question:Where is the company?
Answer:Huarong District, Ezhou City, Hubei Province, is adjacent to Wuhan City.Welcome to visit the company!

Question:What is the delivery time?
Answer:It is determined based on the inventory status of the product and the production and delivery status on that day. Generally, if there is stock available, shipment will be arranged on the day of placing the order.

Question:How to choose the transportation method?
Answer:Determine based on the urgency of the customer's purchase of products and needs. When the quantity of products is low and the value is high, it is recommended to choose air freight or international express delivery; When there is a large quantity of products, choosing sea or land transportation is more cost-effective.

Question:What are the payment methods available?
Answer:T/T,LC, etc

Question:What are the regular packaging options?
Answer:500g/bottle, plastic bottle or 25kg/bucket, cardboard bucket.