Tops Chromogenic Substrate CAS Number 40567-80-4 Trinder′s Reagent

• CAS No.: 40567-80-4
• Formula: C12h18nnao3s
• EINECS: Non
• Classification: Biochemical Reagents
• Grade: AR
• Specific Usage: For Biological Purpose, Pro Analysis
• Model NO.: TOPS
• Content: Standard
• Usage: Laboratory Reagents, Analytical Reagents, Diagnostic Reagents
• Source: Dry Powder
• Habit Appellation: Fine Chemicals
• Application: Industry, Scientific Research, Health
• Property: Biochemical Reagent
• OEM Packing: Yes
• Purity: 99%
• Ivd Reagent: Yes
• Delivery Time: 5-7days
• Storage Conditions: Protected From Light and Moisture
• Colour: White Powder
• CAS Number: 40567-80-4
• Molecular Formula: C12h20nnao3s
• Transport Package: Bottle
• Specification: 50g/bottle
• Trademark: Desheng
• Origin: China
• HS Code: 2921499090

Product Description

Product Description

 

The Chinese full name of TOPS (CAS No. 40567-80-4) is N-ethyl-N - (3-sulfopropyl) -3-methylaniline sodium salt. It is a white to slightly brown powder belonging to the color reagent family. When used as a water-soluble reagent, it can be determined by enzymatic spectrophotometry for hydrogen peroxide. TOPS has been widely used due to its good water solubility, high sensitivity, and strong stability.

 

Product Parameters

 

Apperance:	White Crystal Powder	Purity:	>99%
Molecular Weight:	281.35	Molecular Formula:	C12H20NNaO3S
Grade:	Analytical Grade	Storage Condition:	Inert atmosphere,Room Temperature
Transportation Condition:	Room Temperature(25ºC)	CAS:	40567-80-4

 

 

Product advantages

 

Tops has the characteristics of strong stability, high water solubility, and high detection sensitivity. In the presence of hydrogen peroxide and peroxidase, the color source TOPS forms very stable color dyes during the oxidation reaction with 4-aminoantipyrine or 3-methylbenzothiazole sulfone hydrazone (MBTH). It is widely used for diagnostic examinations and biochemical tests, mainly for the determination of uric acid and cholesterol colorimetry. When used as a water-soluble reagent, hydrogen peroxide can be determined using enzymatic photometry.

 

Application

 

TOPS for creatinine detection of renal function:

In the presence of peroxidase, hydrogen peroxide reacts with 4-aminoantipyrine (4-AAP) and TOPS (ESPMT) or HTIB to produce chromogenic products such as quinone imines. The color intensity can be measured by absorbance and is directly proportional to the concentration of creatinine.

TOPS is used for uric acid testing of renal function:

To detect uric acid, uricase catalyzes the complete oxidation of uric acid with oxygen and water to allantoin, carbon dioxide and hydrogen peroxide H2O2. H2O2 reacts with TOPS and 4-AAP under POD catalysis, and TOPS is oxidized into a color product. The absorbance is measured using a spectrophotometer, and the absorbance intensity is proportional to the uric acid content, thus conducting uric acid analysis.

TOPS is used for detecting free fatty acids in liver function:

The free fatty acids (NEFA) in serum samples react with ATP and CoA under the catalysis of acyl CoA synthase to produce acyl CoA (Acyl CoA), AMP, and phosphate (PPi). Acyl CoA is oxidized to enoyl CoA and hydrogen peroxide under the catalysis of acyl CoA oxidase. Finally, hydrogen peroxide reacts with 4-aminoantipyrine and TOPS under the catalysis of peroxidase to form a red quinone imine compound.
 

Related products

 

The New trinder's reagents refer to highly water-soluble aniline derivatives, which are a large category of chromogenic substrates used in in vitro diagnostic kits to detect changes in liver function, kidney function, blood sugar, and blood lipids, thereby reflecting changes in the body. The principle of action of chromogenic substrates is generally to infer the content and activity of the measured substance by measuring the absorbance change of the chromogenic substrate.

The trinder's reaction principle is to mix the new trinder's reagent 4-AAP with the corresponding oxidase and peroxidase to be tested. Due to the specificity of enzyme substances, they catalyze specific substances, and are sensitive to pH values, biological buffering agents need to be added to adjust to the appropriate pH range of enzyme substances. Add the substance to be tested to the above mixture for color reaction, producing a red quinone imine compound. Calculate the value of the tested item based on its absorbance.


 

Company Profile

Hubei New Desheng Material Technology Co., Ltd. specializes in the production of blood collection tube additives, chemiluminescent reagents, biological buffers, chromogenic substrates, enzyme preparations, antigen antibodies and other biochemical reagents. Founded in 2005, Desheng has a professional R&D team and advanced production equipment!

 

FAQ

Question:Who are we?
Answer:We are Hubei Xindesheng Material Technology Co., Ltd., a professional manufacturer of in vitro diagnostic reagent raw materials.

Question:Where is the company?
Answer:Huarong District, Ezhou City, Hubei Province, is adjacent to Wuhan City.Welcome to visit the company!

Question:What is the delivery time?
Answer:It is determined based on the inventory status of the product and the production and delivery status on that day. Generally, if there is stock available, shipment will be arranged on the day of placing the order.

Question:How to choose the transportation method?
Answer:Determine based on the urgency of the customer's purchase of products and needs. When the quantity of products is low and the value is high, it is recommended to choose air freight or international express delivery; When there is a large quantity of products, choosing sea or land transportation is more cost-effective.

Question:What are the payment methods available?
Answer:T/T,LC, etc

Question:What are the regular packaging options?
Answer:500g/bottle, plastic bottle or 25kg/bucket, cardboard bucket.