Alkaline Optimum High Quality Enzyme Preparation with Low Price

• Customization: Available
• CAS No.: 9001-78-9
• Formula: No
• Model NO.: Alkaline Optimum
• EINECS: Ec:3.1.3.1
• Classification: Enzyme Preparation
• Grade: AR
• Specific Usage: For Biological Purpose
• Content: Standard
• Usage: Laboratory Reagents, Analytical Reagents, Diagnostic Reagents
• Source: Composition
• Habit Appellation: Enzyme Preparation
• Application: Scientific Research, Health
• Property: Biochemical Reagent
• Storage: Low Temperature Storage
• Use to: in Vitro Diagnostic Kit
• Manufactor: Hubei New Desheng Material Technology Co., Ltd
• Dosage Form: White Freeze-Dried Powder
• Molecular Weight: About 35kda
• Transport Package: Refrigerated Transport
• Specification: 10ku/bottle
• Trademark: DeSheng
• Origin: Ezhou City, Hubei Province, China
• Production Capacity: 50g Per Month

Product Description

 

Product Description

The product is for scientific research only and cannot be used for other purposes. Alkaline phosphatase is an enzyme capable of hydrolyzing a series of phosphate compounds. It has the ability to decompose various phosphate molecules through its enzymatic activity. This protein acts as an alkaline phosphatase, catalyzing the hydrolysis of phosphate esters in alkaline environments. Its enzymatic activity enables it to cleave phosphate groups from molecules such as nucleotides, proteins, and phospholipids, rendering them inactive. This multifunctional enzyme plays a crucial role in various physiological processes, including bone mineralization, cell signaling, and metabolism. T3-3AP is a non-specific phosphodiesterase (EC 3.1.3.1) that catalyzes the hydrolysis of phosphate monoesters and exhibits high reactivity towards chromogenic substrates pNPP and luminescent substrates (PPD, APS-5). Suitable for high-sensitivity detection in immunoassays.

 

Product Parameters

Dosage form:	White freeze-dried powder	Molecular weight:	About35kDa (SDS-PAGE detection)
Save buffer:	Tris buffer, pH 7.5	Purity	≥ 90% (SDS-PAGE detection)
Enzyme activity:	60 U/mg	Transportation Conditions:	Low temperature, ice bag
Source:	Gene recombination expression	Safety tips:	Not applicable for human experiments
Storage condition	Freeze dried powder should be stored at 4 ºC for one year and -20 ºC for two years (it is recommended to store separately and avoid repeated freeze-thaw as much as possible)
Vitality unit	Under the conditions of pH 7.5 and 37 ° C, catalytic oxidation of acetyl CoA occurs every minute to produce 1 μ The amount of enzyme required for H2O2 in mol is defined as 1 U unit.

 

The relationship between enzyme preparations, new Trinder's reagents, and Good's buffer

There are many principles of in vitro diagnostic kits, and the most common one is a diagnostic kit based on the Trinder reaction principle (also known as enzyme method), which can be used for the detection of uric acid, creatinine, blood sugar, cholesterol, triglycerides, etc. in blood tests. This diagnostic kit typically includes important components such as enzymes, new Trinder's reagents, and Good's buffer.

By adding the new Trinder's reagent to the test substance, a color reaction occurs, and the value of the test item is calculated through absorbance measurement. For example, the values of blood sugar and uric acid in serum. The new Trinder's reagent has the advantages of high water solubility, UV absorption of color reaction products>540nm, wide pH range requirements for color reactions, and high sensitivity for color reactions.
 

The use of enzyme preparations in the Trinder reaction is due to the presence of too many substances in the serum, and the specificity of enzyme preparations. For example, adding glucose oxidase will only catalyze the oxidation of blood sugar to generate hydrogen peroxide, without catalyzing the oxidation of uric acid and other substances present in the serum, ensuring the accuracy of the detection results as much as possible. However, pH value has a significant impact on enzyme activity, and each enzyme has its own high activity pH range. At this point, Good's buffer is needed to adjust the pH value.

Different Good's buffers can provide suitable buffer intervals for different enzymes. Good's buffer has the advantages of high water solubility, bottom cell membrane permeability, stable acid-base dissociation constant, low metal chelating ability, high chemical stability, etc. In summary, enzyme preparations, new Trinder's reagents, and Good's buffer complement each other in the diagnostic kit, making corresponding contributions to the precise detection of diseases.
 

Company Introduction
Hubei New Desheng Material Technology Co., Ltd. specializes in the production of blood collection tube additives, chemiluminescent reagents, biological buffers, chromogenic substrates, enzyme preparations, antigen antibodies and other biochemical reagents. Founded in 2005, Desheng has a professional R&D team and advanced production equipment!