3-Morpholinopropane Sulfonic Acid Mops Biological Buffer CAS# 1132-61-2

• CAS No.: 1132-61-2
• Formula: C7h15no4s
• EINECS: 214-478-5
• Classification: Biochemical Reagents
• Grade: AR
• Specific Usage: For Biological Purpose, For Tissue Medium Purpose, For Microbiological, For Microscopic Purpose
• Model NO.: Mops Buffer
• Content: Standard
• Usage: Laboratory Reagents, Analytical Reagents, Diagnostic Reagents, Teaching Reagents
• Source: Dry Powder
• Habit Appellation: Fine Chemicals
• Application: Industry, Scientific Research, Health, Environmental Protection
• Property: Biochemical Reagent
• English Name: 3-Morpholinopropane Sulfonic Acid
• Abbreviation: Mops
• Molecular Weight: 209.26
• Molecular Formula: C7h15no4s
• Function: pH Adjustment
• Scope: Good′s Buffers
• Colour: White
• Storage Conditions: Room Temp
• Form: Crystalline Powder
• Water: Solubility Soluble
• Transport Package: 25kgs Per Drum
• Specification: Purity-99.5%
• Trademark: DESHENG
• Origin: Ezhou, China
• HS Code: 2934999099
• Production Capacity: 50tons/Month

Product Description

3-morpholinopropane sulfonic acid MOPS Biological Buffer CAS# 1132-61-2

(English name)3-Morpholinopropane sulfonic acid
(Chinese name)3-Morpholinopropane sulfonic acid
(Abbreviation)MOPS
(CAS#)1132-61-2
(Molecular weight)209.26
(Molecular formula)C7H15NO4S
(Storage condition)room temperature, away from light and moisture
(Molecular structure)
(Application)Mops is a kind of biological buffer, which is often used to prepare RNA electrophoresis buffer.
(Product advantage)The purity is ≥99%, with good water solubility and stable process, which can ensure that the product appearance is pure white crystal powder.
MOPS (3-(N-morpholino)propanesulfonic acid) is a buffer introduced in the 1960s, one of the twenty Good's buffers. It is a structural analog to MES,^[1] and like MES, its structure contains a morpholine ring. HEPES is a similar pH buffering compound that contains a piperazine ring. With a pK_a of 7.20, MOPS is an excellent buffer for many biological systems at near-neutral pH.

Applications

MOPS is frequently used as a buffering agent in biology and biochemistry. It has been tested and recommended for polyacrylamide gel electrophoresis.^[2] Usage above 20 mM in mammalian cell culture work is not recommended.^[3] MOPS buffer solutions become discolored (yellow) over time, but reportedly slight discoloration does not significantly affect the buffering characteristics.^[4]
 

MOPS Buffer (10X) (0.2 M, pH 7) Preparation and Recipe

MOPS buffer is often used in polyacrylamide gel electrophoresis.

 

To prepare  L of MOPS Buffer (10X) (0.2 M, pH 7):
Change the value in the textbox above to scale the recipe volume

 

Table 1. Required components

Component	Amount	Concentration
MOPS free acid (mw: 209.26 g/mol)	41.86 g	0.2 M
Sodium Acetate (mw: 82.03 g/mol)	4.1 g	0.05 M
Disodium EDTA (mw: 372.24 g/mol)	3.72 g	0.01 M

1. Prepare 800 mL of dH2O in a suitable container.
2. Add 41.86 g of MOPS free acid to the solution.
3. Add 4.1 g of Sodium Acetate to the solution.
4. Add 3.72 g of Disodium EDTA to the solution.
5. Adjust solution to desired pH using NaOH (typical pH = 7)
6. Add dH2O until the volume is 1 L.