(English name)3-Morpholinopropane sulfonic acid
(Chinese name)3-Morpholinopropane sulfonic acid
(Abbreviation)MOPS
(CAS#)1132-61-2
(Molecular weight)209.26
(Molecular formula)C7H15NO4S
(Storage condition)room temperature, away from light and moisture
| Biological buffer |
MOPS buffer |
| Primitive trait |
White powder |
| Function |
Used for protein electrophoresis experiments, etc |
| Advantage |
High purity, good water solubility, etc |
| Manufacturer |
Desheng, Hubei |
(Application)Mops is a kind of biological buffer, which is often used to prepare RNA electrophoresis buffer.
(Product advantage)The purity is ≥99%, with good water solubility and stable process, which can ensure that the product appearance is pure white crystal powder.
MOPS (3-(N-morpholino)propanesulfonic acid) is a buffer introduced in the 1960s, one of the twenty Good's buffers. It is a structural analog to MES,[1] and like MES, its structure contains a morpholine ring. HEPES is a similar pH buffering compound that contains a piperazine ring. With a pKa of 7.20, MOPS is an excellent buffer for many biological systems at near-neutral pH.
Applications
MOPS is frequently used as a buffering agent in biology and biochemistry. It has been tested and recommended for polyacrylamide gel electrophoresis.[2] Usage above 20 mM in mammalian cell culture work is not recommended.[3] MOPS buffer solutions become discolored (yellow) over time, but reportedly slight discoloration does not significantly affect the buffering characteristics.[4]
MOPS Buffer (10X) (0.2 M, pH 7) Preparation and Recipe
MOPS buffer is often used in polyacrylamide gel electrophoresis.
To prepare L of MOPS Buffer (10X) (0.2 M, pH 7):
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
- Prepare 800 mL of dH2O in a suitable container.
- Add 41.86 g of MOPS free acid to the solution.
- Add 4.1 g of Sodium Acetate to the solution.
- Add 3.72 g of Disodium EDTA to the solution.
- Adjust solution to desired pH using NaOH (typical pH = 7)
- Add dH2O until the volume is 1 L.
Hubei New Desheng Material Technology Co., Ltd. specializes in the production of blood collection tube additives, chemiluminescent reagents, biological buffers, chromogenic substrates, enzyme preparations, antigen antibodies and other biochemical reagents. Founded in 2005, Desheng has a professional R&D team and advanced production equipment!
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