Hepbs Buffer Composition Is Commonly Used to Stabilize The pH Value of Cell Growth Environment in Cell Culture Media

• CAS No.: 161308-36-7
• Formula: C10h22n2o4s
• EINECS: 629-081-6
• Classification: Biochemical Reagents
• Grade: AR
• Specific Usage: For Biological Purpose, For Tissue Medium Purpose, Technical Grade, Pratical Use, Pro Analysis, Biological Buffer
• Model NO.: Hepbs Buffer
• Content: Purity Above 99%
• Usage: Laboratory Reagents, Diagnostic Reagents
• Source: Dry Powder
• Habit Appellation: Hepbs Buffer
• Application: Scientific Research, Health
• Property: Biochemical Reagent
• Colour: White
• Storage Conditions: Room Temp
• Form: Powder
• Water Solubility: Soluble
• Purity: Above 99%
• Molecular Weight: 266.36
• Density: 1.251±0.06 g/cm3(Predicted)
• pH Value Range: 7.6-9.0
• Pka: 8.3(at 25ºC)
• Use to: Biological Buffer
• Transport Package: Carton
• Specification: 500g/bottle
• Trademark: DeSheng
• Origin: Ezhou City, Hubei Province, China
• Production Capacity: 5 Tons Per Month

Product Description

Packaging Specification : Plastic bottles, 500g/bottle

Basic Information
  

CAS Number	161308-36-7
Molecular Formula	C10H22N2O4S
Molecular Weight	266.36
EINECS	629-081-6
Appearance	White Powder
Density	1.251±0.06 g/cm3(Predicted)
Melting Point	8.3(at 25ºC)
Storage conditions	Inert atmosphere,Room Temperature

 

 
Physical properties of HEPBS

The CAS number of HEPBS is 161308-36-7, and it is a white crystalline powder at 25 ºC. Its pKa value is 8.3, which is in line with the environment of cell growth. The HEPBS produced by our company is reagent grade, and its aqueous solution is slightly turbid. It needs to be prepared and used immediately. In practical applications, titrated with sodium hydroxide solution as a buffer, its UV absorbance is less than 0.1 in a 33% W/W sodium hydroxide solution

Chemical properties of HEPBS

The buffer capacity of HEPBS buffer is strong within the pH range of 7.6 to 9.0. However, it is greatly affected by temperature, so when using HEPBS as a buffer, the influence of biochemical reaction temperature should be considered. HEPBS is a facultative ionic compound with both positive and negative charges in its molecular structure. Its chemical structure contains piperazine ring, which is inactive in chemical property, does not affect the activity of enzymes, does not react with most metals to produce sediment, and does not change the activity coefficient of biochemical system. However, it can be oxidized by strong oxidants and reduced by strong reducing agents.
 

 
Application of HEPBS

HEPBS is a member of the piperazine buffer family, which can coexist with cations in cell culture medium and provide the acidity environment required for cell growth. It is commonly used to stabilize the pH value of cell growth environment in cell culture medium. Due to its high pKa value and suitability for use on the skin, like EPPS, HEPBS can serve as a buffer in cosmetic raw materials to regulate the acidity and alkalinity of various components in cosmetics.



Company Introduction
Hubei New Desheng Material Technology Co., Ltd. specializes in the production of blood collection tube additives, chemiluminescent reagents, biological buffers, chromogenic substrates, enzyme preparations, antigen antibodies and other biochemical reagents. Founded in 2005, Desheng has a professional R&D team and advanced production equipment!