Cystationine Beta Lyase, Cbl, The Core Raw Material of The Cystationine HCl Reagent Kit

Product Description

Basic information
 

Dosage form:	Pale yellow clear liquid or lyophilized powder	Molecular weight:	43kDa(SDS-PAGE detection)
ph stability:	pH7.0-9.0	Purity	≥ 90% (SDS-PAGE detection)
Enzyme activity:	500 U/mg	Transportation Conditions:	Low temperature, ice bag
Source:	Gene recombination expression	Safety tips:	Not applicable for human experiments
storage condition:	Store at 4 ºC for one year (after packaging according to dosage, store at -20 ºC or -80 ºC for at least two years, and try to avoid repeated freeze-thaw)
Definition of enzyme activity:	Under fixed reaction conditions at 37 ºC and pH 8.0, the catalytic reaction results in a 0.01 decrease in the absorbance of NADH at 340nm per minute, defined as an enzyme activity unit.

Product Introduction

Cysteine sulfide β Cystationine beta lyase (CBL EC4.4.1.8) belongs to the class of lyases that catalyze cysteine sulfide β After the removal of pyruvate and NH3, homocysteine is generated through cleavage. This enzyme is used in enzyme cycle methodology for the detection of homocysteine in the blood.




The relationship between enzyme preparations, new Trinder's reagents, and Good's buffer

There are many principles of in vitro diagnostic kits, and the most common one is a diagnostic kit based on the Trinder reaction principle (also known as enzyme method), which can be used for the detection of uric acid, creatinine, blood sugar, cholesterol, triglycerides, etc. in blood tests. This diagnostic kit typically includes important components such as enzymes, new Trinder's reagents, and Good's buffer.

By adding the new Trinder's reagent to the test substance, a color reaction occurs, and the value of the test item is calculated through absorbance measurement. For example, the values of blood sugar and uric acid in serum. The new Trinder's reagent has the advantages of high water solubility, UV absorption of color reaction products>540nm, wide pH range requirements for color reactions, and high sensitivity for color reactions.
 

The use of enzyme preparations in the Trinder reaction is due to the presence of too many substances in the serum, and the specificity of enzyme preparations. For example, adding glucose oxidase will only catalyze the oxidation of blood sugar to generate hydrogen peroxide, without catalyzing the oxidation of uric acid and other substances present in the serum, ensuring the accuracy of the detection results as much as possible. However, pH value has a significant impact on enzyme activity, and each enzyme has its own high activity pH range. At this point, Good's buffer is needed to adjust the pH value.

Different Good's buffers can provide suitable buffer intervals for different enzymes. Good's buffer has the advantages of high water solubility, bottom cell membrane permeability, stable acid-base dissociation constant, low metal chelating ability, high chemical stability, etc. In summary, enzyme preparations, new Trinder's reagents, and Good's buffer complement each other in the diagnostic kit, making corresponding contributions to the precise detection of diseases.
 

Company Introduction
Hubei New Desheng Material Technology Co., Ltd. specializes in the production of blood collection tube additives, chemiluminescent reagents, biological buffers, chromogenic substrates, enzyme preparations, antigen antibodies and other biochemical reagents. Founded in 2005, Desheng has a professional R&D team and advanced production equipment!