Customization: | Available |
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CAS No.: | 1185-53-1 |
Formula: | C4h12clno3 |
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Tris HCl, also known as Tris hydroxymethyl aminomethane hydrochloride. Tris HCl buffer solution is not only widely used as a solvent for nucleic acids and proteins, but also has many important applications.
CAS Number | 1185-53-1 |
Molecular Formula | C4H12ClNO3 |
Molecular Weight | 157.6 |
EINECS | 214-684-5 |
Appearance | White Powder |
Melting Point | 150-152 °C |
Storage conditions | room temp |
Tris HCl is used for protein crystal growth under different pH conditions. The low ionic strength of Tris HCL buffer can be used to form the intermediate fibers of the chemicalbook of the lamin of C. elegans. Tris HCl is also one of the main components of protein electrophoresis buffer. In addition, Tris HCL is also an intermediate for preparing surfactants, vulcanization accelerators, and some drugs. Tris HCl is also used as a titration standard.
A Tris HCl buffer solution with a concentration of 6.057g/L and pH=8.8 was prepared by adjusting the pH value of Chemicalbook using trihydroxymethylaminomethane hydrochloride as the solute and deionized water as the solvent.
1. Trihydroxymethylaminomethane hydrochloride has good solubility in saline water, high solubility in water, and clear liquid;
Each buffer developed has a specific buffer capacity and buffer range. Buffer capacity is the amount of acid or base that can be added before a significant change in pH begins. It can also be defined as the amount of strong acid or strong base that must be added to change the pH value of one liter of solution by one pH unit. After adding acidic or alkaline components, it plays a role in resisting pH changes. Maintain a relatively stable pH value of the solution by neutralizing a small amount of added acid or base.
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