Tris hcl is a neutral buffering agent with a CAS number of 1185-53-1 and a buffer range of 7.5-8.5. However, it itself contains weak alkalinity. Commonly used in various biochemical experiments such as molecular biology and cell biology. Its conventional characteristic is a white powder substance, which is prepared by adding distilled water during use, adjusting to the required pH value, and then being sterilized under high pressure before being stored and used. It is worth noting that if the solution needs to be stored for a long time, it should be stored below -4 ºC. However, in order to better ensure the stability of the product, researchers suggest on-site configuration during use.
| CAS Number |
1185-53-1 |
| Molecular Formula |
C4H12ClNO3 |
| Molecular Weight |
157.6 |
| EINECS |
214-684-5 |
| Appearance |
White Powder |
| Melting Point |
150-152 °C |
| Storage conditions |
room temp |
1) Good solubility, high solubility in water, clear liquid;
2) High purity, up to 99%, stable performance when used;
3) Not precipitating with some metal ions (such as calcium and magnesium);
4) The ion strength of Tris hcl buffer is low;
5) Compared with traditional phosphate buffering agents, Tris hcl is more effective in enzyme determination.
1) Tris hcl is widely used as a protein, nucleic acid, and cell culture buffer;
2) Can be used as a protein crystal growth buffer;
3) Combine with glycine in the electrophoresis buffer to form a buffer system, which is used to stabilize the pH value during the electrophoresis process;
4) Due to its low ion strength, it can be used for the formation of intermediate fibers in nematode nuclear layer proteins;
5) Used for titrating standard substances;
6) Used for the preparation of surfactants.
Each buffer developed has a specific buffer capacity and buffer range. Buffer capacity is the amount of acid or base that can be added before a significant change in pH begins. It can also be defined as the amount of strong acid or strong base that must be added to change the pH value of one liter of solution by one pH unit. After adding acidic or alkaline components, it plays a role in resisting pH changes. Maintain a relatively stable pH value of the solution by neutralizing a small amount of added acid or base.
Desheng is a manufacturer of industrial grade biological buffering agents, currently producing dozens of buffering agents that can be used in various industries.
Hubei New Desheng Material Technology Co., Ltd. specializes in the production of blood collection tube additives, chemiluminescent reagents, biological buffers, chromogenic substrates, enzyme preparations, antigen antibodies and other biochemical reagents. Founded in 2005, Desheng has a professional R&D team and advanced production equipment!
Question:Who are we?
Answer:We are Hubei Xindesheng Material Technology Co., Ltd., a professional manufacturer of in vitro diagnostic reagent raw materials.
Question:Where is the company?
Answer:Huarong District, Ezhou City, Hubei Province, is adjacent to Wuhan City.Welcome to visit the company!
Question:What is the delivery time?
Answer:It is determined based on the inventory status of the product and the production and delivery status on that day. Generally, if there is stock available, shipment will be arranged on the day of placing the order.
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Answer:Determine based on the urgency of the customer's purchase of products and needs. When the quantity of products is low and the value is high, it is recommended to choose air freight or international express delivery; When there is a large quantity of products, choosing sea or land transportation is more cost-effective.
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Answer:T/T,LC, etc
Question:What are the regular packaging options?
Answer:500g/bottle, plastic bottle or 25kg/bucket, cardboard bucket.
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