I. Principle of procoagulation
As blood contains some coagulation factors, procoagulants can play a role in promoting the process of thrombin and
Fibrin clot production process, accelerate the contraction of blood clots, so that the purpose of rapid separation of serum can be achieved.
| Appearance |
White crystal powder |
| Place of Origin |
Ezhou City, Hubei Province |
| Absorbance |
260nm≤0.10 |
| Dissolution |
Easily soluble in water |
| Titer |
150IU/mg |
| Main use |
Used as an external anticoagulant for blood |
II. Test indicators
1, the initial clotting time: observe the initial addition of procoagulant to the test tube and blood samples in contact with the situation, which is roughly fresh proteogen into fresh protein, the appearance of jelly, has been solidified but shaking can move.
2, deep clotting time: shaking to see, there is a clearly visible clot to prove that has been deep clotting.
3,Complete clotting: the blood collection tube will be shaken and observed as immobile.
4,Centrifugation on the machine: centrifugation conditions set to 8 minutes, speed 3200r/min, centrifugal force 1830, after the end of centrifugation to observe whether the appearance of transparent, there is no hemolytic changes.
Use matters
1. The precondition for achieving the coagulation effect has a great relationship with the ambient temperature, so it should be noted that if the blood collection temperature is very low, the amount of procoagulant added and the number of mixing should be adjusted accordingly to give sufficient coagulation time, or with the use of a water bath to change the ambient temperature.
2. Ensure adequate clotting and do not carry out separation before complete coagulation, as this will easily cause haemolysis to occur, or destroy the blood cell components and prevent the separation of a high standard serum sample.
3, pay attention to the centrifugation conditions in the process of centrifugation on the machine, centrifugation is not appropriate, will cause secondary precipitation of fibrin filaments, floating in the serum, it is easy to cause the probe blockage.
As a professional manufacturer of coagulant, Desheng not only has its own unique research and development experience, and more based on the creation of quality products, keep pace with the market, the current production of coagulant has been a hot product, no adverse feedback received from customers. If you have relevant needs, welcome to consult.
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