Title: tris (hydroxymethyl) aminomethane
(English name)Trometamol
(Chinese name)Tris-( hydroxymethyl)-aminometh
(Abbreviation)Tris
(CAS#)77-86-1
(Molecular weight)121.135
(Molecular formula)C4H11NO3
(Storage condition)room temperature, away from light and moisture
(Appearance)white crystal powder
(Molecular structure)
(Application)the product can be used for the preparation of buffer solution in biochemistry and molecular biology experiment, pharmaceutical intermediate, surfactant and curing accelerator, water-soluble polymer containing Tris structure unit as paint dispersant, formaldehyde adsorption material in the room, etc.
| English name |
Trometamol |
| Chinese name |
Tris- hydroxymethyl-aminometh |
| Abbreviation |
Tris |
| CAS# |
77-86-1 |
| Molecular weight |
121.135 |
| Molecular formula |
C4H11NO3 |
| Storage condition |
room temperature, away from light and moisture |
| Appearance |
white crystal powder |
Main applications of Tris
1,Used in biological buffers
In experimental research and analysis, Tris is the buffer used more after phosphate buffer, usually together with acid to ensure the pH value of the reaction system, very common Tris HCL buffer, Tris acetate buffer, etc.. In the past, in vitro diagnostic tests, phosphate buffer will be applied, but because of the involvement of calcium and enzymes, it is easy to generate precipitation, and then choose to use Tris, so that it will not precipitate, but also to ensure the buffering effect.
2,For protein purification
In experimental research, the molecular mechanism of life activities and disease occurrence can be further clarified by protein, so at this time protein extraction is one of the basic and key links. Because it can play a role in maintaining the protein PH value, to ensure that the basic spatial structure of the protein does not change, and has the characteristics of preventing denaturation, so it has become the main buffer.
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