Alpha-Glucosidase Diagnostic Enzyme for in Vitro Diagnostic Kit

Product Description

Product Description

Alpha glucosidase plays a crucial role in the absorption process of food, and food must bind to this enzyme in order to be digested and absorbed. Alpha glucosidase inhibitors slow down the absorption of glucose in the intestine and reduce postprandial hyperglycemia by inhibiting alpha glucosidase on the intestinal mucosa. Since it does not stimulate insulin levels and does not cause hypoglycemia, it helps to reduce blood sugar fluctuations and keep blood sugar levels stable throughout the day.
 

 

Product Parameters

 

Dosage form:	White Powder	Purity:	≥ 90% (SDS-PAGE detection)
Compared to live:	≥10 units/mg protein (using p-nitrophenyl α-D-glucoside as substrate.)	Transportation Conditions:	Low temperature, ice bag
Source:	Gene recombination expression	Safety tips:	Not applicable for human experiments
storage condition:	Store at 4 ºC for about a year (after packaging according to dosage, store at -20 ºC or -80 ºC for a longer period of time and try to avoid repeated freeze-thaw)

 

Product Performance

 

1. Reducing the glycated hemoglobin of patients without increasing weight, and using it in combination with other types of drugs without adverse effects, is beneficial for achieving dual therapeutic effects.

2. It has affinity for the sugars contained in the body, which can slow down the release of polysaccharides in the intestine. For patients, it effectively inhibits the rapid rise of blood sugar after eating, especially suitable for the elderly.

3. Compared with other drugs for treating carbohydrates, such as sulfonylurea drugs, it can effectively reduce the incidence of cardiovascular disease and the risk of liver and kidney diseases.

4. Effectively improving gut microbiota, increasing intestinal peristalsis, reducing fat absorption and accumulation, and during the entire conversion process in the human body, alpha glucosidase does not cause significant side effects.

 

The relationship between enzyme preparations, new Trinder's reagents, and Good's buffer

There are many principles of in vitro diagnostic kits, and the most common one is a diagnostic kit based on the Trinder reaction principle (also known as enzyme method), which can be used for the detection of uric acid, creatinine, blood sugar, cholesterol, triglycerides, etc. in blood tests. This diagnostic kit typically includes important components such as enzymes, new Trinder's reagents, and Good's buffer.

By adding the new Trinder's reagent to the test substance, a color reaction occurs, and the value of the test item is calculated through absorbance measurement. For example, the values of blood sugar and uric acid in serum. The new Trinder's reagent has the advantages of high water solubility, UV absorption of color reaction products>540nm, wide pH range requirements for color reactions, and high sensitivity for color reactions.
 

The use of enzyme preparations in the Trinder reaction is due to the presence of too many substances in the serum, and the specificity of enzyme preparations. For example, adding glucose oxidase will only catalyze the oxidation of blood sugar to generate hydrogen peroxide, without catalyzing the oxidation of uric acid and other substances present in the serum, ensuring the accuracy of the detection results as much as possible. However, pH value has a significant impact on enzyme activity, and each enzyme has its own high activity pH range. At this point, Good's buffer is needed to adjust the pH value.

Different Good's buffers can provide suitable buffer intervals for different enzymes. Good's buffer has the advantages of high water solubility, bottom cell membrane permeability, stable acid-base dissociation constant, low metal chelating ability, high chemical stability, etc. In summary, enzyme preparations, new Trinder's reagents, and Good's buffer complement each other in the diagnostic kit, making corresponding contributions to the precise detection of diseases.
 

Company Introduction
Hubei New Desheng Material Technology Co., Ltd. specializes in the production of blood collection tube additives, chemiluminescent reagents, biological buffers, chromogenic substrates, enzyme preparations, antigen antibodies and other biochemical reagents. Founded in 2005, Desheng has a professional R&D team and advanced production equipment!