Glutamate Dihydrogenase Gldh for Liver Function Testing Kit

• Customization: Available
• CAS No.: No
• Formula: No
• Model NO.: GLDH
• Classification: Biological Agents
• Grade: AR
• Specific Usage: For Biological Purpose
• Content: Standard
• Usage: Laboratory Reagents, Analytical Reagents, Diagnostic Reagents
• Source: Composition
• Habit Appellation: S-Adenosylmethionine Synthetase Sams
• Application: Scientific Research, Health
• Property: Biochemical Reagent
• Storage: Low Temperature Storage
• Use to: in Vitro Diagnostic Kit
• Manufactor: Hubei New Desheng Material Technology Co., Ltd
• Dosage Form: White Freeze-Dried Powder
• Transport Package: Refrigerated Transport
• Specification: 10ku/bottle
• Trademark: DeSheng
• Origin: Ezhou City, Hubei Province, China
• Production Capacity: 50g Per Month

Product Description

Product Description

 

Glutamate dehydrogenase (GLDH, EC1.4.1.2) is an enzyme present in the mitochondrial matrix of cells that can convert glutamate into α- Ketoglutaric acid can also catalyze reverse reactions. GLDH is a type of allosteric enzyme that is produced in the body through reactions such as oxidative dehydrogenation, transamination, co dehydrogenation, and non oxidative dehydrogenation, among which co dehydrogenation is the most important.

 

Product Parameters

 

Dosage form:	White freeze-dried powder, or liquid	Molecular weight:	65kDa(SDS-PAGE detection)
Save buffer:	Tris Cl buffer, pH 8.0	Purity	≥ 90% (SDS-PAGE detection)
Enzyme activity:	300 U/mg	Transportation Conditions:	Low temperature, ice bag
Source:	Gene recombination expression	Safety tips:	Not applicable for human experiments
Vitality unit:	Tris Cl buffer, pH 8.0, pH 8.3, at 37 ºC, requires 1 unit of enzyme to consume 1 umol of NADH per minute.
Storage condition:	Freeze dried powder should be stored at 4 ºC for one year and -20 ºC for two years (it is recommended to store separately and avoid repeated freeze-thaw as much as possible)

 

Application

 

Glutamate dehydrogenase (GLDH) is used for in vitro quantitative determination of the content and activity of glutamate dehydrogenase in biological samples such as serum, plasma, urine, liver tissue, other body fluids, etc.

1. Liver function test kit
Purpose: To detect GLDH activity in serum and evaluate liver function.
Clinical significance: GLDH mainly exists in the mitochondria of liver cells, and when liver cells are damaged, GLDH is released into the bloodstream. Therefore, GLDH activity in serum can serve as a marker of liver cell damage. Commonly used for detecting and evaluating various liver diseases, such as acute and chronic hepatitis, cirrhosis, liver cancer, etc.

2. Bacterial infection detection kit
Usage: Used for detecting Clostridium difficile infection.
Clinical significance: Clostridium difficile can produce GLDH, and detecting GLDH activity can serve as a marker for Clostridium difficile infection. GLDH testing is often combined with other testing methods, such as toxin testing, to improve diagnostic accuracy. Commonly used for the diagnosis of hospital acquired infections and antibiotic associated diarrhea.

Product Performance

 

1.Specificity: GLDH diagnostic enzymes have high specificity and only catalyze specific substrate reactions. Highly specific enzymes reduce non-specific reactions and improve the accuracy of detection. GLDH has high specificity for the substrate L-glutamic acid and can catalyze its oxidative deamination reaction, producing α - ketoglutarate and ammonia.

2.Reversibility: The reaction catalyzed by GLDH is reversible, and α - ketoglutaric acid can be reduced to glutamic acid in the presence of NAD (P) H.

3.Applicability: GLDH diagnostic enzyme is suitable for various detection methods, such as colorimetric method, fluorescence method, and enzyme-linked immunosorbent assay (ELISA). The applicability of multiple detection methods makes GLDH diagnostic enzymes have broad application prospects in different experiments and clinical applications.

4.Reaction rate: GLDH has a high catalytic rate and can catalyze a large amount of substrates for reaction in a short period of time.
 

Precautions

 

Storage conditions: Freeze dried powder should be stored at 4 ºC for one year and at -20 ºC for two years (it is recommended to store separately and avoid repeated freeze-thaw cycles as much as possible).

This reagent is only used for research and development or production, and is strictly prohibited from being used in human or animal experiments

 

 

Company Profile

 

Hubei New Desheng Material Technology Co., Ltd. specializes in the production of blood collection tube additives, chemiluminescent reagents, biological buffers, chromogenic substrates, enzyme preparations, antigen antibodies and other biochemical reagents. Founded in 2005, Desheng has a professional R&D team and advanced production equipment!