Desheng Produces Good Quality Uricase, Uo for Total Cholesterol Kit

Product Description

Basic information
 

Dosage form:	White freeze-dried powder, or liquid	Molecular weight:	35-40kDa(SDS-PAGE detection)
Save buffer:	PBS,pH7.4	Purity	≥ 90% (SDS-PAGE detection)
Enzyme activity:	10 U/mg,dry powder	Transportation Conditions:	Low temperature, ice bag
Source:	Gene recombination expression	Safety tips:	Not applicable for human experiments
Vitality unit:	Oxidize 1% per minute at pH 8.5 and 37 ºC μ The amount of enzyme required for mol uric acid is defined as 1 U unit.
Storage condition:	Freeze dried powder should be stored at 4 ºC for one year and -20 ºC for two years (it is recommended to store separately and avoid repeated freeze-thaw as much as possible)

Product Introduction

Gene Recombinant Protein Description: Uricase (EC 1.7.3.3), also known as uric acid oxidase, is extracted from fermentation broth of Aspergillus niger, Aspergillus flavus, and other fungi. It is a white freeze-dried powder or a gray white to brownish green crystalline or shiny transparent sheet, slightly soluble in alkaline buffer, with a pH value of 6.3. The enzyme activity is inhibited by cyanide, and inhibitors such as Ag+and Hg2+.





The relationship between enzyme preparations, new Trinder's reagents, and Good's buffer

There are many principles of in vitro diagnostic kits, and the most common one is a diagnostic kit based on the Trinder reaction principle (also known as enzyme method), which can be used for the detection of uric acid, creatinine, blood sugar, cholesterol, triglycerides, etc. in blood tests. This diagnostic kit typically includes important components such as enzymes, new Trinder's reagents, and Good's buffer.

By adding the new Trinder's reagent to the test substance, a color reaction occurs, and the value of the test item is calculated through absorbance measurement. For example, the values of blood sugar and uric acid in serum. The new Trinder's reagent has the advantages of high water solubility, UV absorption of color reaction products>540nm, wide pH range requirements for color reactions, and high sensitivity for color reactions.
 

The use of enzyme preparations in the Trinder reaction is due to the presence of too many substances in the serum, and the specificity of enzyme preparations. For example, adding glucose oxidase will only catalyze the oxidation of blood sugar to generate hydrogen peroxide, without catalyzing the oxidation of uric acid and other substances present in the serum, ensuring the accuracy of the detection results as much as possible. However, pH value has a significant impact on enzyme activity, and each enzyme has its own high activity pH range. At this point, Good's buffer is needed to adjust the pH value.

Different Good's buffers can provide suitable buffer intervals for different enzymes. Good's buffer has the advantages of high water solubility, bottom cell membrane permeability, stable acid-base dissociation constant, low metal chelating ability, high chemical stability, etc. In summary, enzyme preparations, new Trinder's reagents, and Good's buffer complement each other in the diagnostic kit, making corresponding contributions to the precise detection of diseases.
 

Company Introduction
Hubei New Desheng Material Technology Co., Ltd. specializes in the production of blood collection tube additives, chemiluminescent reagents, biological buffers, chromogenic substrates, enzyme preparations, antigen antibodies and other biochemical reagents. Founded in 2005, Desheng has a professional R&D team and advanced production equipment!