Factory Direct Sales Color Substrate White Powder Da67

• CAS Number: 115871-18-6
• Molecular Formula: C19h23n4nao3s
• Molecular Weight: 410.47
• Content: Purity Above 99%
• Colour: White Powder
• Water Solubility: Soluble
• Model NO.: Da67
• Use to: Determination of Peroxidase
• Manufacturer: Hubei Xindesheng
• OEM Packing: Yes
• Transport Package: Carton
• Specification: 10g/bottle
• Trademark: DeSheng
• Origin: Ezhou City, Hubei Province, China
• Production Capacity: 500g Per Month

Product Description

Product information

The chromogenic substrate DA-67 is an intermediate metabolite in the constant component analysis and detection of organisms. Its CAS number is 115871-18-6, and its appearance is white powder. It is worth noting that its storage requires temperature, and it needs to be stored at 2-10 ºC. Therefore, if it is transported at normal temperature, it needs to be placed in ice bags and packed in foam boxes.

 

 

Basic information
 

pperance:	White Crystal Powder	Purity:	>99%
Molecular Weight:	410.47	Molecular Formula:	C19H23N4NaO3S
Grade:	Analytical Grade	Storage Condition:	Store at 2-10 ºC,away from light and moisture
Transportation Condition:	Store at 2-10 ºC	CAS:	115871-18-6

 

 

Method for Quantitative Determination of Hydrogen Peroxide Using DA-67

1. Prepare 4.08 mg of DA-67 powder, 1 mL100 units/mL of peroxidase, 0.1mol/L, pH=7, Triton X-100 0.5% PIPES buffer;

2. Place the prepared DA-67 powder and peroxidase into PIPES buffer and bring to a constant volume of 100 mL (DA67 100 μ Mol/L solution);

3. Take 3ml of the prepared solution and mix it with 10 μ Mix the sample solution L (H2O2: 0-5 mmol/L);

4. Incubate at 37 ºC for 5 minutes;

5. Measure the absorbance at 666 nm using a spectrophotometer, and use the absorbance difference of the blank (solution mixed with distilled water to replace the sample solution) as the detection value. Use a separately prepared calibration curve to determine the content in the sample.

The Application Principle of DA-67

When used in combination with peroxidase, DA-67 exhibits high sensitivity to hydrogen peroxide and is a water-soluble oxidative chromogenic reagent. Compared to traditional color reagents, it not only has the characteristics of low absorbance and high sensitivity, but also has better water solubility compared to other color reagents DA-67. At a concentration of more than 1 mmol/L, 10 milligrams can be dissolved in 25 milliliters of water. It is compatible with the above advantages and requires a simple colorimetric method to obtain results quickly, greatly increasing detection efficiency. Therefore, DA-67 is commonly used in the analysis of biological component samples.
 

Company Introduction

Hubei New Desheng Material Technology Co., Ltd. specializes in the production of blood collection tube additives, chemiluminescent reagents, biological buffers, chromogenic substrates, enzyme preparations, antigen antibodies and other biochemical reagents. Founded in 2005, Desheng has a professional R&D team and advanced production equipment!