Desheng Specializes in Producing Lactatedehydrogenase Ldh with High Purity and Good Activity

• CAS No.: No
• Formula: No
• EINECS: No
• Classification: Biological Agents
• Grade: AR
• Specific Usage: For Biological Purpose
• Model NO.: DS0221
• Content: Standard
• Usage: Laboratory Reagents, Analytical Reagents, Diagnostic Reagents
• Source: Composition
• Habit Appellation: Lactatedehydrogenase Ldh
• Application: Scientific Research, Health
• Property: Biochemical Reagent
• Storage: Low Temperature Storage
• Use to: in Vitro Diagnostic Kit
• Manufactor: Hubei New Desheng Material Technology Co., Ltd
• Dosage Form: White Freeze-Dried Powder
• Transport Package: Refrigerated Transport
• Specification: 10mg/bottle
• Trademark: DeSheng
• Origin: Ezhou City, Hubei Province, China
• Production Capacity: 50g Per Month

Product Description

Basic information
 

Dosage form:	White freeze-dried powder, or liquid	Molecular weight:	About40kDa (SDS-PAGE detection)
Save buffer:	Tris Cl buffer, pH 7.5	Purity	≥ 90% (SDS-PAGE detection)
Enzyme activity:	200U/mg protein	Transportation Conditions:	Low temperature, ice bag
Source:	Gene recombination expression	Safety tips:	Not applicable for human experiments
storage condition:	Store at 4 ºC for about a year (after packaging according to dosage, store at -20 ºC or -80 ºC for a longer period of time and try to avoid repeated freeze-thaw)

Product Introduction

Lactate dehydrogenase (LDH, EC1.1.1.27) is a glycolytic enzyme composed of four subunits with a molecular weight of 130-140kDa. It exists in the cytoplasm of all histiocyte of the body, with a higher content in the kidney. There are six forms of LDH isoenzymes that can be separated by electrophoresis, and their distribution has significant tissue specificity. LDH can catalyze the reversible conversion of pyruvate and lactic acid. It is an important enzyme involved in anaerobic glycolysis and gluconeogenesis. The normal range of lactate dehydrogenase in human serum is 135-215U/L. When endocrine disorders or excessive fatigue occur, lactate dehydrogenase levels can be low and can be repaired by conditioning; High lactate dehydrogenase is caused by pleural and ascitic fluid caused by pulmonary infarction, pernicious anemia, shock, and tumor metastasis, which is more severe at this time.




The relationship between enzyme preparations, new Trinder's reagents, and Good's buffer

There are many principles of in vitro diagnostic kits, and the most common one is a diagnostic kit based on the Trinder reaction principle (also known as enzyme method), which can be used for the detection of uric acid, creatinine, blood sugar, cholesterol, triglycerides, etc. in blood tests. This diagnostic kit typically includes important components such as enzymes, new Trinder's reagents, and Good's buffer.

By adding the new Trinder's reagent to the test substance, a color reaction occurs, and the value of the test item is calculated through absorbance measurement. For example, the values of blood sugar and uric acid in serum. The new Trinder's reagent has the advantages of high water solubility, UV absorption of color reaction products>540nm, wide pH range requirements for color reactions, and high sensitivity for color reactions.
 

The use of enzyme preparations in the Trinder reaction is due to the presence of too many substances in the serum, and the specificity of enzyme preparations. For example, adding glucose oxidase will only catalyze the oxidation of blood sugar to generate hydrogen peroxide, without catalyzing the oxidation of uric acid and other substances present in the serum, ensuring the accuracy of the detection results as much as possible. However, pH value has a significant impact on enzyme activity, and each enzyme has its own high activity pH range. At this point, Good's buffer is needed to adjust the pH value.

Different Good's buffers can provide suitable buffer intervals for different enzymes. Good's buffer has the advantages of high water solubility, bottom cell membrane permeability, stable acid-base dissociation constant, low metal chelating ability, high chemical stability, etc. In summary, enzyme preparations, new Trinder's reagents, and Good's buffer complement each other in the diagnostic kit, making corresponding contributions to the precise detection of diseases.
 

Company Introduction
Hubei New Desheng Material Technology Co., Ltd. specializes in the production of blood collection tube additives, chemiluminescent reagents, biological buffers, chromogenic substrates, enzyme preparations, antigen antibodies and other biochemical reagents. Founded in 2005, Desheng has a professional R&D team and advanced production equipment!