Desheng Specializes in Producing Lactatedehydrogenase Ldh with High Purity and Good Activity

Product Details
Customization: Available
CAS No.: No
Formula: No
Manufacturer/Factory & Trading Company

360° Virtual Tour

Diamond Member Since 2023

Suppliers with verified business licenses

Audited Supplier

Audited by an independent third-party inspection agency

Importers and Exporters
The supplier has import and export rights
Patents Awarded
The supplier had awarded 2 patents, you can check the Audit Report for more information
Exhibition Experience
The supplier had participated in offline trade shows, you can check the Audit Report for more information
to see all verified strength labels (16)
  • Desheng Specializes in Producing Lactatedehydrogenase Ldh with High Purity and Good Activity
  • Desheng Specializes in Producing Lactatedehydrogenase Ldh with High Purity and Good Activity
  • Desheng Specializes in Producing Lactatedehydrogenase Ldh with High Purity and Good Activity
  • Desheng Specializes in Producing Lactatedehydrogenase Ldh with High Purity and Good Activity
  • Desheng Specializes in Producing Lactatedehydrogenase Ldh with High Purity and Good Activity
Find Similar Products

Basic Info.

Model NO.
DS0221
EINECS
No
Classification
Biological Agents
Grade
AR
Specific Usage
For Biological Purpose
Content
Standard
Usage
Laboratory Reagents, Analytical Reagents, Diagnostic Reagents
Source
Composition
Habit Appellation
Lactatedehydrogenase Ldh
Application
Scientific Research, Health
Property
Biochemical Reagent
Storage
Low Temperature Storage
Use to
in Vitro Diagnostic Kit
Manufactor
Hubei New Desheng Material Technology Co., Ltd
Dosage Form
White Freeze-Dried Powder
Transport Package
Refrigerated Transport
Specification
10mg/bottle
Trademark
DeSheng
Origin
Ezhou City, Hubei Province, China
Production Capacity
50g Per Month

Product Description

Basic information
 
Dosage form: White freeze-dried powder, or liquid Molecular weight: About40kDa (SDS-PAGE detection)
Save buffer: Tris Cl buffer, pH 7.5 Purity ≥ 90% (SDS-PAGE detection)
Enzyme activity: 200U/mg protein Transportation Conditions: Low temperature, ice bag
Source: Gene recombination expression Safety tips: Not applicable for human experiments
storage condition: Store at 4 ºC for about a year (after packaging according to dosage, store at -20 ºC or -80 ºC for a longer period of time and try to avoid repeated freeze-thaw)

Product Introduction

Lactate dehydrogenase (LDH, EC1.1.1.27) is a glycolytic enzyme composed of four subunits with a molecular weight of 130-140kDa. It exists in the cytoplasm of all histiocyte of the body, with a higher content in the kidney. There are six forms of LDH isoenzymes that can be separated by electrophoresis, and their distribution has significant tissue specificity. LDH can catalyze the reversible conversion of pyruvate and lactic acid. It is an important enzyme involved in anaerobic glycolysis and gluconeogenesis. The normal range of lactate dehydrogenase in human serum is 135-215U/L. When endocrine disorders or excessive fatigue occur, lactate dehydrogenase levels can be low and can be repaired by conditioning; High lactate dehydrogenase is caused by pleural and ascitic fluid caused by pulmonary infarction, pernicious anemia, shock, and tumor metastasis, which is more severe at this time.

Desheng Specializes in Producing Lactatedehydrogenase Ldh with High Purity and Good Activity


The relationship between enzyme preparations, new Trinder's reagents, and Good's buffer


There are many principles of in vitro diagnostic kits, and the most common one is a diagnostic kit based on the Trinder reaction principle (also known as enzyme method), which can be used for the detection of uric acid, creatinine, blood sugar, cholesterol, triglycerides, etc. in blood tests. This diagnostic kit typically includes important components such as enzymes, new Trinder's reagents, and Good's buffer.

By adding the new Trinder's reagent to the test substance, a color reaction occurs, and the value of the test item is calculated through absorbance measurement. For example, the values of blood sugar and uric acid in serum. The new Trinder's reagent has the advantages of high water solubility, UV absorption of color reaction products>540nm, wide pH range requirements for color reactions, and high sensitivity for color reactions.

 
Desheng Specializes in Producing Lactatedehydrogenase Ldh with High Purity and Good Activity
Desheng Specializes in Producing Lactatedehydrogenase Ldh with High Purity and Good Activity
Desheng Specializes in Producing Lactatedehydrogenase Ldh with High Purity and Good Activity


The use of enzyme preparations in the Trinder reaction is due to the presence of too many substances in the serum, and the specificity of enzyme preparations. For example, adding glucose oxidase will only catalyze the oxidation of blood sugar to generate hydrogen peroxide, without catalyzing the oxidation of uric acid and other substances present in the serum, ensuring the accuracy of the detection results as much as possible. However, pH value has a significant impact on enzyme activity, and each enzyme has its own high activity pH range. At this point, Good's buffer is needed to adjust the pH value.

Different Good's buffers can provide suitable buffer intervals for different enzymes. Good's buffer has the advantages of high water solubility, bottom cell membrane permeability, stable acid-base dissociation constant, low metal chelating ability, high chemical stability, etc. In summary, enzyme preparations, new Trinder's reagents, and Good's buffer complement each other in the diagnostic kit, making corresponding contributions to the precise detection of diseases.

 
Desheng Specializes in Producing Lactatedehydrogenase Ldh with High Purity and Good Activity
Desheng Specializes in Producing Lactatedehydrogenase Ldh with High Purity and Good Activity
Desheng Specializes in Producing Lactatedehydrogenase Ldh with High Purity and Good Activity

Company Introduction
Hubei New Desheng Material Technology Co., Ltd. specializes in the production of blood collection tube additives, chemiluminescent reagents, biological buffers, chromogenic substrates, enzyme preparations, antigen antibodies and other biochemical reagents. Founded in 2005, Desheng has a professional R&D team and advanced production equipment!

 
Desheng Specializes in Producing Lactatedehydrogenase Ldh with High Purity and Good Activity
Desheng Specializes in Producing Lactatedehydrogenase Ldh with High Purity and Good Activity
Desheng Specializes in Producing Lactatedehydrogenase Ldh with High Purity and Good Activity
 
Desheng Specializes in Producing Lactatedehydrogenase Ldh with High Purity and Good Activity
Desheng Specializes in Producing Lactatedehydrogenase Ldh with High Purity and Good Activity
Desheng Specializes in Producing Lactatedehydrogenase Ldh with High Purity and Good Activity

Send your message to this supplier

*From:
*To:
*Message:

Enter between 20 to 4,000 characters.

This is not what you are looking for? Post a Sourcing Request Now
Contact Supplier