CAS 77-86-1 Tris (Hydroxymethyl) Aminometh Ane Tham Tris Base

• CAS No.: 77-86-1
• Formula: C4h11no3
• Classification: High Purity Material
• Grade: AR
• Specific Usage: For Biological Purpose
• Content: Standard
• Model NO.: Tris base
• Usage: Laboratory Reagents, Analytical Reagents
• Habit Appellation: Good′s Buffer
• Application: Industry
• Colour: White
• Form: Crystalline Powder
• Molecular Weight: 121.135
• Storage Temperature: Room Temperature
• Storage Conditions: Avoid Light and Moisture
• Purity: Above 99%
• Product Advantages: Good Water Solubility
• Use to: Biological Buffer
• OEM Packing: Yes
• Transport Package: 500g / Bottle, 25kg / Barrel
• Trademark: DESHENG
• Origin: China
• HS Code: 2922199090
• Production Capacity: 100t/Month

Product Description

 

Principle and Application of Biological Buffer Tris

In biological experiments, biological buffers should be used frequently, and Tris is a commonly used type in biological buffers. As a widely used biological buffer, many people should know this name, but some people still don't understand it. Let's take a look at the principle and application of Tris.
 

Configuration of Tris buffer solution

{Product Name}:	Tris Base
{Other Name}:	Tris/Tris(hydroxymethyl)amino eth ane/THAM/Trometamol
{CAS No.}:	77-86-1
{Einecs No.}:	201-064-4
{Appearance}:	White Crystalline Powder
{Speccification}:	99%min
{Molecular formula}:	C4H11NO3
{MOQ}:	1kg
{Application}:	Chemical Reagents
{Brand}:	MANSON

 

PH value required(25ºC)	7.10	7.20	7.30	7.40	7.50	7.60	7.70	7.80	7.90	8.00	8.10	8.20	8.30	8.40	8.50	8.60	8.70	8.80	8.90
0.1mol/L HCI volume	45.7	44.7	43.4	42	40.3	38.5	36.6	34.5	32	29.2	26.2	22.9	19.9	17.2	14.7	12.4	10.3	8.5	7

Tris is called trishydroxymethylaminome thane, also called tromethamine, with CAS number 77-86-1, and its appearance is white crystalline powder. It is soluble in ethanol and water. It also dissolves with ethyl  acetate but is relatively slow. It does not react with ether or carbon  tetrachloride. It is corrosive to copper and aluminum.

 

 

Tris is widely used in acute metabolic and respiratory acidemia. It is an alkaline buffering agent. It has a good buffering effect on metabolic acidosis and enzyme activity. It may cause hypoglycemia, hypotension, nausea, and vomiting. It can even inhibit breathing and stop breathing. Excessive intake or renal insufficiency can cause alkalemia. When Tris and acid titration standard materials are used, the purity requirements will be very high, basically 99.9%-100%

Because Tris is weakly alkaline, when the temperature is below 25ºC, Pka is 8.1, but according to the buffer theory, its effective buffer range is between PH7.0-9.2. The pH of the aqueous solution of Tris is about 10.5, and hydrochloric  acid is generally used to adjust the pH to the desired value, but at the same time, attention should be paid to the effect of temperature on the Pka of Tris. Since the Tris buffer is a weakly alkaline solution, DNA will be deprotonated in the solution, thereby improving its solubility. Therefore, people often add EDTA to Tris hydrochloric  acid buffer to make TE buffer, and change the pH adjustment solution to acetic acid to obtain TAE buffer.

 

Desheng Biotechnology has been established for decades and has 19 years of experience in the development and production of biological buffers. Tris is the company's first batch of R&D products and is the company's core product. It has been sold to more than one hundred worldwide. Country, product quality is reliable, price concessions can be negotiated, interested can call for consultation, Desheng Biotechnology welcomes your calls.