3-morpholinopropane sulfonic acid MOPS Biological Buffer CAS# 1132-61-2
(English name)3-Morpholinopropane sulfonic acid
(Chinese name)3-Morpholinopropane sulfonic acid
(Abbreviation)MOPS
(CAS#)1132-61-2
(Molecular weight)209.26
(Molecular formula)C7H15NO4S
(Storage condition)room temperature, away from light and moisture
(Molecular structure)
(Application)Mops is a kind of biological buffer, which is often used to prepare RNA electrophoresis buffer.
(Product advantage)The purity is ≥99%, with good water solubility and stable process, which can ensure that the product appearance is pure white crystal powder.
MOPS (3-(N-morpholino)propanesulfonic acid) is a buffer introduced in the 1960s, one of the twenty Good's buffers. It is a structural analog to MES,[1] and like MES, its structure contains a morpholine ring. HEPES is a similar pH buffering compound that contains a piperazine ring. With a pKa of 7.20, MOPS is an excellent buffer for many biological systems at near-neutral pH.
Applications
MOPS is frequently used as a buffering agent in biology and biochemistry. It has been tested and recommended for polyacrylamide gel electrophoresis.[2] Usage above 20 mM in mammalian cell culture work is not recommended.[3] MOPS buffer solutions become discolored (yellow) over time, but reportedly slight discoloration does not significantly affect the buffering characteristics.[4]
MOPS Buffer (10X) (0.2 M, pH 7) Preparation and Recipe
MOPS buffer is often used in polyacrylamide gel electrophoresis.
To prepare L of MOPS Buffer (10X) (0.2 M, pH 7):
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
Component |
Amount |
Concentration |
MOPS free acid (mw: 209.26 g/mol) |
41.86 g |
0.2 M |
Sodium Acetate (mw: 82.03 g/mol) |
4.1 g |
0.05 M |
Disodium EDTA (mw: 372.24 g/mol) |
3.72 g |
0.01 M |
- Prepare 800 mL of dH2O in a suitable container.
- Add 41.86 g of MOPS free acid to the solution.
- Add 4.1 g of Sodium Acetate to the solution.
- Add 3.72 g of Disodium EDTA to the solution.
- Adjust solution to desired pH using NaOH (typical pH = 7)
- Add dH2O until the volume is 1 L.