Tris (hydroxymethyl) Aminomethane Hydrochloride Biological Buffer CAS#1185-53-1

• CAS No.: CAS#1185-53-1
• Formula: C4h12clno3
• Classification: Biochemical Reagents
• Grade: AR
• Specific Usage: For Biological Purpose, For Tissue Medium Purpose, For Microbiological, For Microscopic Purpose
• Content: Standard
• Model NO.: Tris HCl
• Usage: Laboratory Reagents, Analytical Reagents, Diagnostic Reagents, Teaching Reagents
• Source: Dry Powder
• Habit Appellation: Fine Chemicals
• Application: Industry, Scientific Research, Health
• Property: Biochemical Reagent
• Product Name: Tris(Hydroxymethyl)Aminomethane Hydrochloride
• Abbreviation: Tris-HCl
• CAS#: 1185-53-1
• Molecular Formula: C4h12clno3
• Appearance: White Crystal Powder
• Molecular Weight: 157.6
• Use to: Biological Buffer
• Manufacturer: Hubei Xindesheng
• OEM Packing: Yes
• Transport Package: 25kgs Per Carton Drum
• Specification: Purity-99.5%
• Trademark: DESHENG
• Origin: Ezhou, China
• HS Code: 382200900
• Production Capacity: 1000kgs/Month

Product Description

Tris(hydroxymethyl)aminomethane Hydrochloride
(Chinese name)Tris(hydroxymethyl)aminomethane Hydrochloride
(Abbreviation)Tris-HCl
(CAS#)1185-53-1
(Molecular weight)157.6
(Molecular formula)C4H12ClNO3
(Storage condition)room temperature, away from light and moisture
(Appearance)white crystal powder
(Molecular structure)

(Application) the product can be used for the preparation of buffer solution in biochemistry and molecular biology experiment, pharmaceutical intermediate, surfactant and curing accelerator, water-soluble polymer containing Tris structure unit as paint dispersant, formaldehyde adsorption material in the room, etc.

(Product advantage) purity ≥ 99%, good water solubility, stable process.
 

>99% (titration), pH 7.0-9.0, suitable for FISH

Synonym(s):

Trizma^® hydrochloride, TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride

Linear Formula:

NH₂C(CH₂OH)₃ · HCl
 

PROPERTIES

Assay

>99% (titration)

form

solid

packaging

pkg of 500 g

manufacturer/tradename

Roche

technique(s)

FISH: suitable

pH

7.0-9.0

useful pH range

7.0-9.0

pKa (25 °C)

8.1

absorption

<0.02 at 300 nm at 100 mg/mL

storage temp.

room temp

SMILES string

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

InChI key

QKNYBSVHEMOAJP-UHFFFAOYSA-N

Short for Tris (hydroxymethyl) aminomethane (THAM) hydrochloride, Tris HCl is an organic compound often used in buffer solutions such as TAE or TBE for electrophoresis gels. Tris is highly soluble in water and is useful in the pH range 7.0-9.0. It is used in the preparation of Laemmli buffer, one of the most common SDS-PAGE buffers. In addition, Tris can also be used for many custom running and loading buffers, most often with glycine and SDS. The use of single-junction pH electrodes that contain silver should be avoided when determining the pH of a solution containing Tris buffer.

A Tris buffer solution can be made by mixing Tris with Tris-HCl. This prevents overshooting the pH and prevents the need to work with strong acids or bases. If neutralizing Tris-HCl with a strong base such as NaOH to make a buffer solution, ionic strength should be taken into consideration because of the formation of salt.

Product Specifications
Tris Hydrochloride
Tris HCl;
2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride

Formula: C₄H₁₁NO₃ · HCl

MW: 157.60 g/mol

Storage/Handling: Store in a dry and well-ventilated place.

PubChem Chemical ID: 93573

PRODUCT SPECIFICATIONS

Catalog ID	DS-CP022
CAS #	1185-53-1
MW	157.60 g/mol
Storage/Handling	Store desiccated at room temperature.

1M Tris HCl Buffer - 1 L Instructions
1. Dissolve 157.60 g of Tris HCl (Tris HCl, CAS 1185-53-1, mw. = 157.60 g/mol]) in 750 mL of dH2O.
2. Adjust to desired pH using 10N NaOH. This method will introduce 0.06M - 0.81M NaCl into the concentrated stock solution.
3. Fill to final volume of 1 L with dH2O.
4. Filter sterilize (recommended) or autoclave. 5. Store at 4 deg C.

To make a 1 L solution of 1M Tris HCl, use the table below to estimate the required volume of base for a given pH: Starting pH: 3.58 Adjust pH with: 10N NaOH pH 7.0 7.1 7.2 7.3 7.4 7.5 7.6 7.7 7.8 7.9 8.0 8.1 8.2 8.3 8.4 8.5 8.6 8.7 8.8 8.9 9.0 mL 6 7 9 11 13 16 19 23 27 31 36 41 46 51 56 61 66 70 75 78 81 Note: This data was collected in GoldBio labs using GoldBio reagents and calculated using 100 ml volumes. All reagent volumes recorded above were adjusted accordingly to create this protocol. Tris pKa at 25°: 8.06 Tris pH range: 7.0 - 9.0 d(pKa)/dT value: -0.028